Uridine (Uridin) is a nucleoside compound, one of the four bases that make up RNA, that replaces thymine in DNA during transcription and pairs with adenine.

H9 cells:10 μg/mL, P2Y14 receptor:7.28 (pEC₅₀), P2Y6:300 nM, RAW264.7 cells:> 10 μM (EC50)

方法: HCC 细胞 HEPG2、HLF 和 97H 用 Uridine (0-600 µM) 处理 48 h，使用 CCK8 assay 检测细胞活力。
结果: 三种 HCC 细胞的增殖能力受到显著的剂量依赖性抑制。[1]
方法: 分化的 PC-12 细胞用 Uridine (50 µM) 处理 4 天，使用 Western Blot 检测靶点蛋白表达水平。
结果: Uridine 处理增强分化 PC12 细胞神经丝表达。Uridine 治疗后，NF-M 和 NF-70 的表达均显著增加。[2]

方法: 为检测体内抗肿瘤活性，将 Uridine (100-300 mg/kg) 局部注射给携带 97H 异种移植物的 BALB/c 裸鼠，每天一次，持续十六天。
结果: 两种剂量的 Uridine 均显著降低了 HCC 裸鼠皮下肿瘤的肿瘤大小，且呈剂量依赖性。[1]

Microtubule depolymerizing activity: The effects of 2-Methoxyestradiol on cellular microtubule depolymerization are determined by indirect immunofluorescence techniques in rat aortic smooth muscle A-10 cells. Microtubules are visualized using a β-tubulin antibody. Three viewers determines the percent microtubule loss for each treatment concentration. The data are averaged and plotted as percent microtubule loss versus drug concentration and the EC50s for microtubule depolymerization calculated from the log dose–response curves.